J.bale Bio : Sib piv ntawm inactivated thiab non-inactivated VTM

Kev sib piv ntawm ob VTM: inactivated thiab non-inactivated

VTM (tus kab mob thauj mus los media) yog siv rau kev sau, khaws cia, kev thauj mus los ntawm cov kab mob sib kis, xws li kab mob corona, kab mob khaub thuas, kab mob HFMD, thiab lwm yam. los ntawm caj pas, Nasal swabs lossis lwm qhov tshwj xeeb, cov qauv khaws cia tuaj yeem siv rau kev sim tshuaj tom ntej xws li nucleic acid extraction lossis purification. Feem ntau muab faib ua ob hom, ib qho yog hom tsis muaj zog, uas tuaj yeem tiv thaiv cov protein thiab nucleic acid. ntawm tus kab mob no, thiab lwm yam yog ib hom inactivated, uas feem ntau muaj ib tug lysis ntsev uas inactivates tus kab mob, uas cleavages cov protein thiab tiv thaiv cov nucleic acid.Ob tug muaj lawv tus kheej sib txawv nta thiab txawv daim ntawv thov cov lus qhia.

1. Inactivated virus preservation solution:

Nucleic acid yog cov tshuaj lom neeg macromolecular tsim los ntawm polymerization ntawm ntau nucleotides, suav nrog deoxyribonucleic acid (DNA) thiab ribonucleic acid (RNA). Nws yog ib qho tseem ceeb tshaj plaws ntawm lub neej. Tus kab mob no muaj ib tug qauv thiab tsuas muaj ib hom nucleic acid thiab protein. Yog li, tus kab mob no raug kuaj pom thaum kuaj pom cov nucleic acid. Cov kab mob yog cov kab mob parasitic thiab tsis tuaj yeem muaj sia nyob sab nraum lub cev tom qab kuaj. Yog tias lawv tsis tuaj yeem kuaj tau raws sijhawm, lawv yuav tsum tau muab tso rau hauv cov tshuaj tiv thaiv kab mob. Txhawm rau tiv thaiv kev nyab xeeb ntawm qhov chaw kuaj kab mob, nws yog ib qho tsim nyog yuav tsum tau ntxiv lysis ntsev kom tsis txhob muaj kab mob thiab tso tawm cov nucleic acid uas tuaj yeem kuaj pom.

Inactivated virus preservation solution yog tsuas yog ib tug kab mob lysis preservation tov hloov los ntawm nucleic acid extraction lysis tov, uas yog ntxiv nrog ib tug siab concentration ntawm lysis ntsev, uas tau sai thiab zoo deactivate tus kab mob protein ntawm cov qauv mus kuaj, uas muaj peev xwm tiv thaiv tau zoo. tus neeg teb xov tooj Tus kab mob thib ob, uas tseem muaj Rnase enzyme inhibitor, tuaj yeem tiv thaiv tus kab mob nucleic acid los ntawm kev degraded. Lub hom phiaj tseem ceeb ntawm Tris, lysis ntsev, EDTA, thiab lwm yam uas muaj nyob rau hauv nws yog kom tshem tawm cov nucleic acid thiab tso tawm cov nucleic acid, uas tuaj yeem nqa tawm los ntawm lub sijhawm tiag tiag fluorescent RT-PCR Nucleic acid kuaj yog siv los txiav txim. seb tus qauv twg muaj tus kab mob-featured nucleic acid, uas yog, seb nws puas kis tus kab mob. Cov tshuaj inactivated cia yuav khaws cia rau hauv chav tsev kub rau lub sij hawm ntev, txuag tus nqi ntawm tus kab mob coj mus kuaj cia thiab kev thauj mus los.

Cov yam ntxwv ntawm inactivated virus preservation tov khoom:

1. Kev ua haujlwm yooj yim thiab siv tau yooj yim, tsis tas yuav npaj ua kua, lub kaw lus muaj cov kab mob lysate ua haujlwm siab, tus kab mob no tsis ua haujlwm tam sim tom qab kuaj, tiv thaiv kev pheej hmoo ntawm kev kis kab mob thib ob, thiab ua kom muaj kev nyab xeeb ntawm kev thauj mus los thiab cov neeg ua haujlwm kuaj;

2. Viral DNA/RNA tuaj yeem khaws cia thiab thauj hauv chav sov li 1 lub lis piam yam tsis muaj degradation. Tom qab kev rho tawm raws li cov khoom lag luam feem ntau, DNA / RNA tau txais yog qhov zoo thiab muaj txiaj ntsig zoo, thiab tuaj yeem ua tiav ntau yam kev tshuaj ntsuam genetic tests thiab kev soj ntsuam kev sim. Xws li PCR, qPCR, thiab lwm yam, thaum txuag nqi tsheb thauj mus los;

3. Muaj RNase inhibitors kom txhawb kev tiv thaiv kab mob nucleic acid kom tsis txhob degraded thiab txhim kho kev ua tau zoo ntawm nucleic acid extraction.

4. Nws yog qhov tsim nyog rau kev sau, khaws cia thiab kev thauj mus los ntawm cov qauv kab mob xws li tus kabmob tshiab, tus kab mob khaub thuas, kab mob tes, ko taw thiab qhov ncauj. Nws tuaj yeem sau cov caj pas thiab qhov ntswg qhov ntswg los yog cov ntaub so ntswg kuaj ntawm qhov tshwj xeeb. Cov qauv khaws cia tuaj yeem siv rau kev sim tshuaj tom ntej xws li nucleic acid extraction lossis purification.

2. Cov tshuaj tiv thaiv kab mob uas tsis yog inactivated:

Cov tshuaj tiv thaiv kab mob uas tsis muaj kev tiv thaiv tsis muaj lysis ntsev, tab sis tus kab mob khaws cia muaj kev ncaj ncees zoo dua thiab muaj txiaj ntsig ntau dua. Nws feem ntau yog raws li kev txhim kho kab mob kev tiv thaiv kev tiv thaiv kev daws teeb meem raws li kev xa khoom nruab nrab. Ntxiv nrog rau kev tshawb nrhiav nucleic acid, nws tuaj yeem siv rau lwm yam kev tshawb fawb. Qhov no khaws cov txheej txheem protein ntawm tus kab mob thiab DNA lossis RNA ntawm tus kab mob nucleic acid tib lub sijhawm, kom tus kab mob no muaj kev ncaj ncees ntawm cov protein epitope thiab nucleic acid hauv vitro. Tau kawg, muaj qee qhov kev pheej hmoo ntawm kev kis kab mob thaum ua haujlwm yuam kev. Nws cov khoom xyaw feem ntau muaj Hanks kua hauv paus, gentamicin, tshuaj tua kab mob fungal, BSA(V), cryoprotectants, lom buffers thiab amino acids, thiab lwm yam., uas tiv thaiv tus kab mob protein plhaub los ntawm yooj yim decomposed thiab tswj lub originality ntawm tus kab mob qauv mus rau qhov loj tshaj. qhov twg. Lub sij hawm ntev cia tom qab kuaj yuav tsum ua kom nruj me ntsis kub.

Cov yam ntxwv ntawm cov khoom uas tsis yog inactivated virus preservation tov khoom:

1. Qhov ntsuas kub qis tsis khov tsis ua rau lub plhaub sab nraud ntawm tus kab mob thiab yooj yim rau kev thauj mus los ntev.

2. Haum rau txhua yam ntawm cov qauv swab, nrog rau qhov ncauj swabs, qhov ntswg qhov ntswg, caj pas swabs, thiab lwm yam.

3. Nws tuaj yeem siv rau tus kab mob khaub thuas H1N1 thiab lwm yam kab mob uas tuaj yeem kuaj nrog cov swabs.

4. Cov kab mob nucleic acid tuaj yeem muab rho tawm nrog kab mob kab mob DNAout lossis kab mob kab mob RNAout.

5. Cov tshuaj tua kab mob hauv cov qauv tshuaj tuaj yeem tiv thaiv kab mob thiab cov kab mob fungal.

6. Cov qauv tshuaj ntxiv nrog bovine serum albumin, uas tuaj yeem tiv thaiv tus kab mob tus qauv thiab txhim kho qhov sib cais.

Nws tuaj yeem pom los ntawm kev sib piv ntawm ob qhov uas lub hom phiaj ntawm kev tsim cov khoom siv tsis ua haujlwm yog los tswj kev ncaj ncees ntawm cov kab mob kis kab mob, thiab nws cov muaj pes tsawg leeg yog complex dua li hom inactivated, thiab tus nqi yog me ntsis siab dua. Raws li kev pheej hmoo kis tus kab mob, tsis tas yuav txhawj xeeb. Tsuav yog cov tsev kawm ntawv uas tuaj yeem ua cov tshuaj tua kab mob nucleic acid raug tsim thiab ua haujlwm raws li qhov yuav tsum tau ua, tsis muaj kev phom sij, thiab tseem tsis tau muaj tus kab mob kis los ntawm tus kab mob cia tshuaj los ntawm tus neeg soj ntsuam.

Cov tshuaj tiv thaiv kab mob uas tsis yog inactivated muaj kev xav tau ntau dua rau qhov kev sim, thiab nws qhov kev nkag siab yuav tsum siab dua, thiab muaj ntau daim ntawv thov. Tom qab tag nrho, cov tshuaj khaws cia tau ntxiv rau tus kab mob swab sampling raj rau cov kev pabcuam kuaj kab mob, thiab tsis tuaj yeem yog vim nws kis tau. Tsuas yog tsis lees paub nws, qhov kev pheej hmoo tswj tiag tiag yuav tsum dag hauv kev ua piv txwv thiab kev sim ua haujlwm.


Post lub sij hawm: Jun-09-2021